Rat Malonyl-CoA Decarboxylase; Cloning, Expression in E. coli and its Biochemical Characterization
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منابع مشابه
Rat malonyl-CoA decarboxylase; cloning, expression in E. coli and its biochemical characterization.
Malonyl-CoA decarboxylase (E.C.4.1.1.9) catalyzes the conversion of malonyl-CoA to acetyl-CoA. Although the metabolic role of this enzyme has not been fully defined, it has been reported that its deficiency is associated with mild mental retardation, seizures, hypotonia, cadiomyopathy, developmental delay, vomiting, hypoglycemia, metabolic acidosis, and malonic aciduria. Here, we isolated a cDN...
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Nicolas VOILLEY*, Raphae$ l RODUIT*, Raffaela VICARETTI*, Christophe BONNY†, Ge! rard WAEBER†, Jason R. B. DYCK‡, Gary D. LOPASCHUK‡ and Marc PRENTKI* *Molecular Nutrition Unit, Department of Nutrition, University of Montreal and the CR Centre Hospitalier de l’Universite! de Montre! al and Institut du Cancer, 1560 Sherbrooke Est, Montreal, Quebec H2L 4M1, Canada, †Department of Internal Medicin...
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Malonyl-CoA decarboxylase was purified from goose uropygial gland, reduced, carboxymethylated, and digested with trypsin. Several peptides were purified by high performance liquid chromatography and their amino acid sequences determined. Oligonucleotide probes were prepared based on their amino acid sequences. Size-selected RNA from the goose uropygial gland was used to construct cDNA libraries...
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ژورنال
عنوان ژورنال: BMB Reports
سال: 2002
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2002.35.2.213